期刊
MICROBIOLOGY
卷 82, 期 6, 页码 833-840出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S002626171401007X
关键词
Azospirillum; formation of gold nanoparticles; laccase; Mn peroxidase
类别
Plant-associated nitrogen-fixing soil bacteria Azospirillum brasilense were shown to reduce the gold of chloroauric acid to elemental gold, resulting in formation of gold nanoparticles. Extracellular phenoloxidizing enzymes (laccases and Mn peroxidases) were shown to participate in reduction of Au+3 (HAuCl4) to Au-0. Transmission electron microscopy revealed accumulation of colloidal gold nanoparticles of diverse shape in the culture liquid of A. brasilense strains Sp245 and Sp7. The size of the electron-dense nanospheres was 5 to 50 nm, and the size of nanoprisms varied from 5 to 300 nm. The tentative mechanism responsible for formation of gold nanoparticles is discussed.
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