期刊
MICROBIOLOGY
卷 80, 期 4, 页码 483-487出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026261711040060
关键词
lipase; Bacillus vallismortis; halophilic bacteria; the Maharla Salt Lake
类别
资金
- Research Council of Shiraz University of Medical Sciences, Shiraz, Iran
Lipases are an important class of enzymes which catalyze the hydrolysis of long chain triglycerides and constitute the most prominent group of biocatalysts for biotechnological applications. There are a number of lipases, produced by some halophilic microorganisms. In this study, some lipase producing bacteria from the Maharla salt lake located in south of Iran were isolated. All isolates were screened for true lipase activity on plates containing olive oil. The lipase activity was measured using titrimetric methods. Among thirty three isolates, thirteen strains demonstrating orange zone around colonies under UV light, were selected for identification using the molecular methods and some morphological characteristics. The bacterium Bacillus vallismortis BCCS 007 with 3.41 +/- 0.14 U/mL lipase activity was selected as the highest lipase producing isolate. This is the first report of isolation and molecular identification of lipase producing bacteria from the Maharla lake.
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