期刊
MICROBIOLOGY
卷 80, 期 1, 页码 15-20出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026261711010176
关键词
inorganic polyphosphates; carbon source; aeration; CCCP; yeast; Saccharomyces cerevisiae
类别
资金
- Russian Foundation for Basic Research [08-04-00472]
- Presidium of the Russian Academy of Sciences [RAN P-24]
- Support of Leading Scientific Schools of the Russian Federation [NS-1004.2008.4]
The effects of different concentrations of the protonophore uncoupler m-carbonyl cyanide 3-hchlorophenylhydrazone (CCCP) on the synthesis of inorganic polyphosphates (polyP) during the first 0.5 h of hypercompensation in the yeast Saccharomyces cerevisiae VKM Y-1173 growing on media with 2% glucose under low (hypoxia) or high aeration or with 1% (vol/vol) ethanol under high aeration were studied. It was shown that the yeast growth on ethanol was completely inhibited by 5 mu M CCCP, while growth on glucose was inhibited by 25 mu M CCCP, independently of aeration of the medium. The maximum rate of H-2 absorption was shown at 2, 5, and 25 mu M CCCP for the cells grown on ethanol, on glucose under high aeration, and on glucose under hypoxia, respectively. Against the decrease of total ATP level and total polyP, CCCP had a nonuniform effect on the synthesis of individual polyP fractions. CCCP maximally inhibited synthesis of the most actively formed fractions: polyPI during growth on glucose under hypoxia, polyPIII during growth on glucose under aeration, and polyPIII and polyPV during growth on ethanol. CCCP had no substantial effect on the synthesis of polyPII and polyPIV fractions, the formation of which seems to be less related to the electrochemical potential gradient of H+ ions.
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