期刊
MICROBIOLOGY
卷 77, 期 2, 页码 176-180出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026261708020094
关键词
Pseudomonas; phenazine antibiotics; producers; mutants
类别
N-methyl-N'-nitro-N-nitrosoguanidine (NG)-induced mutagenesis with subsequent selection for resistance to toxic amino acid analogues (azaserine, m-fluoro-DL-phenylalanine, and 6-diazo-5-oxo-L-norleucine) was applied to Pseudomonas aurantiaca B-162. The resulting strains produced phenazine antibiotics three times more efficiently than the wild type strain and ten times more efficiently than the known pseudomonad strains. Overproduction of phenazine antibiotics was shown to result either from deregulation of 3-deoxy-D-arabinohepulosonate-7-phosphate synthase (DAHP synthase), the key enzyme of the aromatic pathway (removal of inhibition by phenylalanine, tyrosine, and phenazine), or overproduction of N-hexanoyl homoserine lactone, the regulatory molecules of positive control of cellular metabolism (QS systems).
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