期刊
MICROBIOLOGICAL RESEARCH
卷 167, 期 10, 页码 616-622出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2012.02.008
关键词
Secondary metabolite; Marine actinomycete BM-17; Spectroscopy
类别
资金
- Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University [JKGQ201102]
- National Nature Science Foundation of China for Young Scientists [81001395]
A marine actinomycete, designated strain BM-17, was isolated from a sediment sample collected in the Arctic Ocean. The strain was identified as Nocardia dassonvillei based on morphological, cultural, physiological, biochemical characteristics, along with the cell wall analysis and 16S rDNA gene sequence analysis. A new secondary metabolite (1), N-(2-hydroxyphenyl)-2-phenazinamine (NHP), and six known antibiotics (2-7) have been isolated from the saline culture broth of the stain by sequentially purification over macroporous resin D101, silica gel, Sephadex LH-20 column chromatography and preparative HPLC after the stain was incubated in soy bean media at 28 degrees C for 7days. The chemical structures of the compounds were elucidated on the basis of spectroscopic analysis, including two-dimensional (2D) NMR and HR-ESI-MS data. The new compound showed significant antifungal activity against Candida albicans, with a MIC of 64 mu g/ml and high cancer cell cytotoxicity against HepG2, A549, HCT-116 and COD cells. (C) 2012 Elsevier GmbH. All rights reserved.
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