期刊
MICROBIOLOGICAL RESEARCH
卷 166, 期 2, 页码 99-110出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2010.02.003
关键词
16S rRNA; Phylogeny; Diversity; Metagenomics; Multilocus sequence analysis
类别
资金
- Department of Science and Technology, New Delhi [0070/2008]
- University Grants Commission through Centre for Excellence in Genomic Sciences
- University Grants Commission through Networking Resource Centre in Biological Sciences
Small subunit ribosomal RNA (16S rRNA) gene sequence analysis is used for the identification and classification of prokaryotes. In addition, sequencing of 16S rRNA genes amplified directly from the environment is used to estimate microbial diversity. The presence of mosaicism, intra-genonnic heterogeneity and the lack of a universal threshold sequence identity value limit 16S rRNA-based phylogenetic analysis. PCR-amplification bias and cloning bias can also result in an inaccurate representation of the microbial diversity. In this review, recently reported complexities of 16S rRNA gene sequence analyses and the requirement of additional tools for microbial phylogeny and diversity analyses are discussed. (C) 2010 Elsevier GmbH. All rights reserved.
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