期刊
MICROBIOLOGICAL RESEARCH
卷 165, 期 8, 页码 665-673出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2009.11.013
关键词
Fire blight; Two-component system; Antimicrobial peptide; Type III secretion
类别
资金
- USDA National Institute of Food and Agriculture (NIFA) [Illu-802-317]
- USDA-NIFA-Agriculture and Food Research Initiative (AFRI)
- Agricultural Experiment Station of Illinois
The PhoPQ system is a pleiotropic two-component signal transduction system that controls many pathogenic properties in several mammalian and plant pathogens. Three different cues have been demonstrated to activate the PhoPQ system including a mild acidic pH, antimicrobial peptides, and low Mg2+. In this study, our results showed that phoPQ mutants were more resistant to strong acidic conditions (pH 4.5 or 5) than that of the wild-type (WT) strain, suggesting that this system in Erwinia amylovora may negatively regulate acid resistance gene expression. Furthermore, the PhoPQ system negatively regulated gene expression of two novel type III secretion systems in E. amylovora. These results are in contrast to those reported for the PhoPQ system in Salmonella and Xanthomonas, where it positively regulates type III secretion system and acid resistance. In addition, survival of phoPQ mutants was about 10-fold lower than that of WT when treated with cecropin A at pH 5.5, suggesting that the PhoPQ system renders the pathogen more resistant to cecropin A. (C) 2009 Elsevier GmbH. All rights reserved.
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