期刊
MICROBIOLOGICAL RESEARCH
卷 165, 期 1, 页码 75-81出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2008.08.002
关键词
Exo-beta-1,3-glucanase; Trichoderma asperellum; Regulation; Mycoparasitism
类别
资金
- CNPq [62.02553/92-4]
- MCT-FINEP [0845/07]
- FUNAPE/UFG
- PIBIC/CNPq
The regulation of the gene encoding the extracellular exo-beta-1,3-glucanase (tag83) produced by the mycoparasite Trichoderma asperellum was studied. Enzyme activity was detected in all carbon sources, but the highest levels were found when starch and purified cell watts from Rhizoctonia solani were used. These results are supported by the appearance of one strong band with enzyme activity in non-denaturing PAGE. Experiments using RT-PCR showed that exo-beta-1,3-glucanase induction in T asperellum occurred at the transcriptional level. We used RT-PCR and real-time PCR analysis to examine the expression of tag83 gene during in vivo assay of T asperellum against R. solani. We showed that the expression of tag83 is significantly increased by the presence of R. solani. (C) 2008 Elsevier GmbH. All rights reserved.
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