期刊
MICROBIOLOGICAL RESEARCH
卷 163, 期 6, 页码 711-716出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2007.12.002
关键词
aiiA gene; Bacillus subtilis; Bioassay; Expression; Sequence analysis
类别
资金
- National Natural Science Foundation of China [30571257]
- Hi-Tech Research and Development Program of China [2006AA10A212]
- SRFDP [20060389011]
- Fujian Province [2006F3016]
- Education Department of Fujian Province [JA07066]
- Natural Science Foundation of Fujian Province [80610005]
AHL-lactonase (AiiA), a metallo-beta-lactamase produced by Bacillus thuringiensis, Bacillus cereus and Bacillus anthracis, specifically hydrolyzes N-acyl-homoserine lactones (AHLs) secreted by Gram-negative bacteria and thereby attenuates the symptoms caused by plant pathogens. In this study, an aiiA gene was cloned from Bacillus subtilis BS-1 by PCR with a pair of degenerate primers. The deduced 250 amino acid sequence contained two small conserved regions, (SHLHFDH109)-S-103 and (166)TPGHTPG(173), which are characteristic of the metallo-beta-lactamase family. Homology comparison revealed that the deduced amino acid sequence had a high degree of similarity with those of the known AiiA proteins in the B. cereus group. Additionally, the aiiA gene was expressed in Escherichia coli BL21 (DE3) pLysS and the expressed AiiA protein could attenuate the soft rot symptoms caused by Erwinia carotovora var. carotovora. (c) 2007 Elsevier GmbH. All rights reserved.
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