期刊
MICROBIAL PATHOGENESIS
卷 51, 期 3, 页码 142-148出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2011.04.007
关键词
Staphylococcus aureus; Extracellular DNA; Nuclease; Colony spreading
资金
- Naito Foundation
- National Institute of Biomedical Innovation (NIBIO)
- Genome Pharmaceuticals Institute
- Grants-in-Aid for Scientific Research [23249009, 11J11070] Funding Source: KAKEN
Staphylococcus aureus spreads on soft agar surfaces and forms giant colonies. Here, we examined the inhibitory role of extracellular DNA on the colony spreading activity. The double-deletion mutation of nuc1 and nuc2, which encode secretory nucleases, increased extracellular DNA and showed a decreased ability to form giant colonies. The addition of DNase I or micrococcal nuclease to the soft agar restored the ability of the nuc1-nuc2 double mutant to form giant colonies. In addition, the promoter activities of nuc1 and nuc2 in the wild-type strain were elevated in the peripheral region of the giant colony. These findings suggest that the digestion of extracellular DNA by secretory nucleases is required for the colony spreading activity of S. aureus. (C) 2011 Elsevier Ltd. All rights reserved.
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