期刊
MICROBIAL PATHOGENESIS
卷 45, 期 2, 页码 98-104出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2007.12.004
关键词
Citrobacter rodentium; Tight junction; Intestinal permeability; Rho kinase; Programmed cell death; Apoptosis-inducing factor
资金
- Natural Sciences and Engineering Research Council of Canada
- Crohn's and Colitis Foundation of Canada
The murine model of Citrobacter rodentium infection has been used to complement in vitro studies of enteropathogenic Escherichia coli (EPEC) infections of human intestinal epithelial cells (IECs). However, the differences in epithelial cell responses between these two models are not fully understood. We used an in vitro model of C. rodentium infection to examine important, yet incompletely understood cellular, responses of murine IECs to this pathogen. C. rodentium attached to CMT-93 cells and disrupted their tight junctional expression of claudins-4 and -5. This was associated with a loss of barrier function that required live bacteria and was partially prevented by the inhibition of Rho kinase. Furthermore, C rodentium caused an upregulation of IEC apoptosis that was associated with the cytoplasmic accumulation of apoptosis-inducing factor, but not with the activation of caspase-3. These studies demonstrate for the first time that C rodentium affects murine IECs in ways that may be similar, but distinct, to the effects of EPEC on human IECs. (c) 2008 Elsevier Ltd. All rights reserved.
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