期刊
MICROBIAL ECOLOGY
卷 66, 期 1, 页码 73-83出版社
SPRINGER
DOI: 10.1007/s00248-013-0224-1
关键词
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资金
- National Science and Technology Major Project of China [2012ZX07101-010]
- National Natural Science Foundation of China [31100021, 51079139]
- Chinese Academy of Sciences [KZCX2-EW-314, 2011T1Z37]
- Open Research Foundation of Nanjing Institute of Geography and Limnology [NIGLAS2010QD12]
- Natural Science Foundation of Jiangsu Province of China [SBK201122829]
The phylogenetic diversity of the microbial community assemblage of the carpet-like mucilaginous cyanobacterial blooms in the eutrophic Lake Taihu was investigated. 16S ribosomal DNA clone libraries produced from the DNA of cyanobacterial assemblages that had been washed to remove unattached bacteria contained only cyanobacteria. However, a further treatment which included grinding the freeze-dried material to physically detach cells followed by the removal of larger cells by filtration allowed us to detect a large variety of bacteria within the cyanobacterial bloom community. Interestingly, the dominant members of the microbial community were Planctomycetes followed by Cytophaga-Flavobacterium-Bacteroides (CFB), Betaproteobacteria, and Gammaproteobacteria. The analysis of the 16S ribosomal DNA clone libraries made from enrichment culture revealed much higher phylogenetic diversity of bacteria. Dominant bacterial groups in the enrichment system were identified as members of the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria subdivisions, CFB group, and Planctomycetes. In addition, the clone libraries constructed from Planctomycetes-specific 16S ribosomal RNA primers also verified that the enrichment allowed a diversity of Planctomycetes to proliferate, although the community composition was altered after enrichment.
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