期刊
MICROBES AND INFECTION
卷 15, 期 1, 页码 74-83出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2012.10.016
关键词
MAG1_4; MAG1_5; Sex-difference; Diagnostic marker; Early immune response
资金
- Stanley Medical Research Institute
To distinguish active from inactive/chronic infection in Toxoplasma gondii-seropositive individuals, we have developed an enzyme-linked immunosorbent assay (ELISA) using specific peptides derived from Toxoplasma matrix antigen MAGI. We used this assay to measure matrix specific antibodies and pilot studies with infected mice established the validity of two peptides. The immune response against MAGI occurs in about 12 days postinfection and displays a sex difference later on in mouse model, with males producing higher antibody titers than females. Serum samples from 22 patients with clinical toxoplasmosis and from 26 patients with serological evidence of past exposure to Toxoplasma (more than one year infection history) were analyzed. Both MAGI peptides detected antibodies significant frequently and robustly from active stage than from the chronic stage of toxoplasmosis. The results indicate that both MAGI peptides may be used as a tool to differentiate active from inactive infection. It also may be considered in the design of potential vaccines in humans. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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