期刊
MICROBES AND INFECTION
卷 15, 期 13, 页码 874-886出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2013.08.002
关键词
Cross-clade; CTL; HLA; Epitope; HIV
资金
- Global COE program Global Education and Research Center Aiming at the control of AIDS
- Institute of Tropical Medicine, Nagasaki University
- Ministry of Education, Science, Sports, and Culture, Japan
- Grants-in-Aid for Scientific Research [25870550, 25870552] Funding Source: KAKEN
Identification of cross-clade T cell epitopes is one of key factors for the development of a widely applicable AIDS vaccine. We here investigated cross-clade CD8+ T cell responses between clade B and A/E viruses in chronically HIV-1 clade A/E-infected Japanese individuals. CD8+ T cell responses to 11-mer overlapping peptides derived from Nef, Gag, and Pol clade B consensus sequences were at a similar level to those to the same peptides found in clade B-infected individuals. Fifteen cross-clade CTL epitopes were identified from 13 regions where the frequency of responders was high in the clade AM-infected individuals. The sequences of 6 epitopes were conserved between the clade B and clade AM viruses whereas 9 epitopes had different amino acid sequences between the 2 viruses. CD8+ T cells specific for the 6 conserved epitopes recognized cells infected with the clade A/E virus, whereas those for 8 diverse epitopes recognized both the clade A/E virus-infected and clade B-infected cells. All of the cross-clade CD8+ T cells specific for conserved and diverse epitopes were detected in chronically HIV-1 clade A/E-infected individuals. These results show that in addition to conserved regions polymorphic ones across the clades can be targets for cross-clade CTLs. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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