期刊
MICROBES AND INFECTION
卷 14, 期 12, 页码 1054-1063出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2012.05.012
关键词
Ehrlichia chaffeensis; Tandem repeat proteins; Immunoreactive proteins; Epitope; Antibody
资金
- National Institute of Allergy and Infectious Diseases (NIAID) [R01 AI 071145, T32 AI060549]
- Clayton Foundation for Research
Humoral immune mechanisms are an important component of protective immunity to Ehrlichia species. However, the molecular basis of antibody mediated immunity is not completely defined, and the role of most molecularly characterized major immunoreactive proteins is unknown. In previous studies, we mapped major species-specific continuous epitopes in three surface exposed and secreted tandem repeat proteins (TRP32, TRP47 and TRP120). In this study, we report that protection is provided by antibodies against these molecularly defined TRP epitopes using vitro and in vivo models. Protection was demonstrated in vitro after prophylactic and therapeutic administration of epitope-specific anti-TRP antibodies, suggesting that the protective mechanisms involve extracellular and intracellular antibody-mediated effects. In vivo passive transfer of individual epitope-specific TRP sera significantly reduced the ehrlichial load and splenomegaly, and protected mice against lethal infection. Moreover, the combination of antibodies to all three TRPs provided enhanced reduction in ehrlichial load similar to that of Ehrlichia chaffeensis immune sera. IgGI was the predominant antibody isotype in the epitope-specific TRP mouse sera. These results demonstrate that antibodies against linear epitopes in TRP32, TRP47 and TRP120 are protective during E. chaffeensis infection and involves extracellular and intracellular antibody-mediated mechanisms. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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