期刊
MICROBES AND INFECTION
卷 14, 期 3, 页码 279-289出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2011.09.006
关键词
Macrophage migration inhibitory factor; Strongyloides; Excretory/secretory proteins; Immune response; Cytokine
资金
- Deutsche Forschungsgemeinschaft (DFG) [BR 1020/4-1, LI 793/5-0]
- Egyptian Ministry of Higher Education
Strongyloidiasis is a tropical parasitosis characterized by an alternation between free-living and parasitic stages, and by long-term infection via autoinfection. Since invasion and evasion processes of helminth parasites are substantially attained by the involvement of excretory secretory products, we identified and characterized the 13.5 kDa macrophage migration inhibitory factor (MIF)-like protein in Strongyloides ratti. Sra-MIF is mainly secreted from the infective stage larvae (iL3), while the transcript was found at lower levels in parasitic and free-living females. Sequence analysis of the full-length cDNA showed the highest homology to the human pathogen Strongyloides stercoralis, and both are related to the MIF type-2. Unlike other mif genes, the Sra-mif includes no intron. The protein was recombinantly expressed in Escherichia coli and purified. Sra-MIF exhibited no in vitro tautomerase activity. The exposure of Sra-MIF to the host immune system is confirmed by high IgG reactivities found in the hosts' sera following infection or immunization. Flow cytometric analysis indicated the binding of Sra-MIF to the monocytes/macrophage lineage but not to peripheral lymphocytes. After exposure to Sra-MIF, monocytes released IL-10 but not TNF-alpha suggesting the involvement of the secreted parasite MIF in host immune responses. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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