期刊
MICROBES AND INFECTION
卷 14, 期 2, 页码 169-176出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2011.09.004
关键词
Japanese encephalitis virus; E protein; NS1; Recombinant proteins; Mucosal vaccine; Adjuvant
资金
- Bio-oriented Technology Research Advancement Institution
- Okinawa Industry Promotion Public Corporation (Naha, Okinawa, Japan)
Ectodomain of Japanese encephalitis virus (JEV) E protein [domains I through III (D1-3), domains I and II (D1-2) and domain III (D3)] and the nonstructural protein I (NS1) were expressed in Escherichia coli, and administered to BALB/c mice via the intranasal (i.n.) route. The E protein, but not the NS1, induced JEV-specific serum IgG with virus-neutralization capacity in vitro. When mice were lethally challenged with JEV, in. immunization with D1-3, D1-2, D3, or a mouse brain-derived formalin-inactivated JE vaccine conferred complete protection, while an 80% protection rate was observed in the NS1 immunized mice. Cytokine analysis of the cervical lymph nodes of mice in. immunized with D1-3 or NS1 revealed antigen-specific IL-2 and IL-17 responses, but no IFN-gamma T cell response, were observed. This study demonstrates for the first time the i.n. vaccine efficacy of the E. coli-expressed recombinant JEV proteins. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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