期刊
MICROBES AND INFECTION
卷 11, 期 2, 页码 215-222出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2008.11.007
关键词
Leishmania; Lipophosphoglycan; Membrane rafts; Phagosomal maturation; Actin
资金
- Swedish Medical Research Council [6251, 13103]
- Swedish Research Council [621-2001-3570, 20073483, 529-2003-5994, 2005-7046, 521-2002-6393]
- Swedish Society for Medical Research
- Swedish Medical Association
- Magn. Bergvalls Stiftelse
- Stiftelsen Lars Hiertas Minne
- Ekhaga Foundation
- Canadian Institutes of Health Research [MOP-12933]
- Foundation Armand-Frappier
Lipophosphoglycan (LPG), the major surface glycoconjugate on Leishmania donovani promastigotes, is crucial for the establishment of infection inside macrophages. LPG comprises a polymer of repeating Gal beta 1,4Man alpha-PO4 attached to a lysophosphatidylinositol membrane anchor. LPG is transferred from the parasite to the host macrophage membrane during phagocytosis and induces periphagosomal F-actin accumulation correlating with an inhibition of phagosomal maturation. The biophysical properties of LPG suggest that it may be intercalated into membrane rafts of the host-cell membrane. The aim of this study was to investigate if the effects of LPG on phagosomal maturation are mediated via action on membrane rafts. We show that LPG accumulates in rafts during phagocytosis of L. donovani and that disruption of membrane rafts abolished the effects of LPG on periphagosomal F-actin and phagosomal maturation, indicating that LPG requires intact membrane rafts to manipulate host-cell functions. We conclude that LPG associates with membrane rafts in the host cell and exert its actions on host-cell actin and phagosomal maturation through subversion of raft function. (C) 2008 Elsevier Masson SAS. All rights reserved.
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