期刊
MICROBES AND ENVIRONMENTS
卷 28, 期 3, 页码 388-390出版社
JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
DOI: 10.1264/jsme2.ME12200
关键词
Colpoda; excystment; DEAD-box RNA helicase
资金
- Sasagawa Scientific Research Grant from the Japan Science Society [24-407]
- Grants-in-Aid for Scientific Research [13J08784] Funding Source: KAKEN
Protein expression during the excystment of Colpoda cucullus was studied by SDS-PAGE. The expression levels of 60-, 50- and 49-kDa proteins were markedly changed from the early to later stage of excystment. The 60-kDa protein (p60) was temporarily expressed first, and its expression was inhibited by actinomycin D. LC-MS/MS analysis showed that the amino acid sequences of p60 partially coincided with those of the Paramecium tetraurelia unnamed protein homologous to DEAD-box RNA helicase. These results suggest that p60 expression is enhanced by transcriptional regulation and may be involved in initiating the molecular events leading to cellular morphogenesis.
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