期刊
METHODS
卷 68, 期 1, 页码 82-88出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.02.018
关键词
RNAi; Functional genomics; High-throughput screening; Drosophila; Cell-based assays; In vivo assays
资金
- NIH NIGMS [R01 GM067761]
- Dana Farber/Harvard Cancer Center - NCI Cancer Center Support Grant [NIH 5 P30 CA06516]
- NIGMS [R01 GM084947]
- NCRR/ORIP [R24 RR032668]
Here, I discuss how RNAi screening can be used effectively to uncover gene function. Specifically, I discuss the types of high-throughput assays that can be done in Drosophila cells and in vivo, RNAi reagent design and available reagent collections, automated screen pipelines, analysis of screen results, and approaches to RNAi results verification. (C) 2014 Published by Elsevier Inc.
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