期刊
METHODS
卷 68, 期 2, 页码 354-363出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.02.006
关键词
Gold nanoparticles; Nanoparticle uptake; Intracellular imaging; Surface-enhanced Raman spectroscopy SERS
资金
- EPSRC [EP/H028757/2] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H028757/2] Funding Source: researchfish
Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies. (C) 2014 Elsevier Inc. All rights reserved.
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