4.7 Article

Screening protein - Single stranded RNA complexes by NMR spectroscopy for structure determination

期刊

METHODS
卷 65, 期 3, 页码 288-301

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.09.018

关键词

NMR spectroscopy; Protein-RNA complex; STAR proteins; Sam68; T-STAR

资金

  1. Medical Research Council Career Development Award [G1000526]
  2. College of Medicine, Biological Sciences and Psychology, University of Leicester
  3. MRC [G1000526] Funding Source: UKRI
  4. Medical Research Council [G1000526] Funding Source: researchfish

向作者/读者索取更多资源

In the past few years, RNA molecules have been revealed to be at the center of numerous biological processes. Long considered as passive molecules transferring genetic information from DNA to proteins, it is now well established that RNA molecules play important regulatory roles. Associated with that, the number of identified RNA binding proteins (RBPs) has increased considerably and mutations in RNA molecules or RBP have been shown to cause various diseases, such as cancers. It is therefore crucial to understand at the molecular level how these proteins specifically recognise their RNA targets in order to design new generation drug therapies targeting protein-RNA complexes. Nuclear magnetic resonance (NMR) is a particularly well-suited technique to study such protein-RNA complexes at the atomic level and can provide valuable information for new drug discovery programs. In this article, we describe the NMR strategy that we and other laboratories use for screening optimal conditions necessary for structural studies of protein-single stranded RNA complexes, using two proteins, Sam68 and T-STAR, as examples. (c) 2013 The Authors. Published by Elsevier Inc. All rights reserved.

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