期刊
METHODS
卷 65, 期 2, 页码 201-206出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.09.012
关键词
Signal transduction; Protein tyrosine phosphatases; Phosphorylation; Posttranslational modification; Sumoylation
资金
- NCI NIH HHS [R01 CA058836] Funding Source: Medline
Signaling enzymes are typically regulated by a variety of post-translational modifications. One such enzyme, protein tyrosine phosphatase (PTP) 1B, which plays an important role in metabolic and growth signaling, has recently been shown to be inhibited by posttranslational modifications by ubiquitin-related proteins of the SUMO family. We have adapted several approaches to analyzing the sites and effects of sumoylation of PTP1B in cells and in vitro. The principal outline of the assays should be applicable to other enzymes as well. (C) 2014 Published by Elsevier Inc.
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