期刊
METHODS
卷 63, 期 1, 页码 41-49出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.06.027
关键词
Algorithms; Tools; RNAseq; NGS; Next-generation sequencing; Sequencing; Adapter trimming; Pipelines
资金
- EU FP7 (SIROCCO) [LSHG-CT-2006-037900]
- BBSRC UK [BB/01589X/1]
- BBSRC [BB/J019275/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J019275/1] Funding Source: researchfish
New sequencing technologies pose significant challenges in terms of data complexity and magnitude. It is essential that efficient software is developed with performance that scales with this growth in sequence information. Here we present a comprehensive and integrated set of tools for the analysis of data from large scale sequencing experiments. It supports adapter detection and removal, demultiplexing of barcodes, paired-end data, a range of read architectures and the efficient removal of sequence redundancy. Sequences can be trimmed and filtered based on length, quality and complexity. Quality control plots track sequence length, composition and summary statistics with respect to genomic annotation. Several use cases have been integrated into a single streamlined pipeline, including both mRNA and small RNA sequencing experiments. This pipeline interfaces with existing tools for genomic mapping and differential expression analysis. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
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