期刊
METHODS
卷 62, 期 1, 页码 13-25出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.03.026
关键词
Transcription; Transcriptional regulation; Stochastic models; Chemical master equation; Single cell experiments
资金
- NSF [DMR-0706458, DMR-1206146]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1206146] Funding Source: National Science Foundation
Genes in prokaryotic and eukaryotic cells are typically regulated by complex promoters containing multiple binding sites for a variety of transcription factors leading to a specific functional dependence between regulatory inputs and transcriptional outputs. With increasing regularity, the transcriptional outputs from different promoters are being measured in quantitative detail in single-cell experiments thus providing the impetus for the development of quantitative models of transcription. We describe recent progress in developing models of transcriptional regulation that incorporate, to different degrees, the complexity of multi-state promoter dynamics, and its effect on the transcriptional outputs of single cells. The goal of these models is to predict the statistical properties of transcriptional outputs and characterize their variability in time and across a population of cells, as a function of the input concentrations of transcription factors. The interplay between mathematical models of different regulatory mechanisms and quantitative biophysical experiments holds the promise of elucidating the molecular-scale mechanisms of transcriptional regulation in cells, from bacteria to higher eukaryotes. (C) 2013 Elsevier Inc. All rights reserved.
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