期刊
METHODS
卷 64, 期 3, 页码 305-314出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.07.004
关键词
Biosensors; Metallointercalators; Nanomaterials; Label-free; Fluorescence
资金
- National Natural Science Foundation of China [20901060, 31170776, 81171646]
- Fundamental Research Funds for the Central Universities
DNA based biosensors have become increasingly important in medical diagnostics, genetic and drug screening, and forensics in this post-genomic era. Chemical labeling methods suffer from several drawbacks, which are tedious, cost-expensive and time-consuming, thus the development of simple and general label free strategies is being demanded. The present article introduces a new model of biosensor device based on both metallointercalators and nanomaterials, with the aim of highlighting, in particular, the use of the label-free strategy for the construction of simple and inexpensive sensing platforms. In this work, two strategies were developed for designing label-free sensors: (1) metallointercalators act as the quencher to nanomaterials; (2) nanomaterials act as the quencher to metallointercalators. These methods take advantage of the sensitive luminescence signal change of metallointercalators or nanomaterials upon analytes binding. By monitoring the luminescence change, we were able to detect metal ions and biomolecules. Compared to other label-free fluorescent methods, these label-free DNA biosensors provide fast, simple, economical, sensitive and selective detection of target analytes with specific probes. (c) 2013 Elsevier Inc. All rights reserved.
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