期刊
METHODS
卷 58, 期 3, 页码 289-299出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2012.08.009
关键词
ChIP; ChIA-PET; Chromatin interactions; Genome; Next-generation sequencing
资金
- A*STAR
- L'Oreal for Women in Science National Fellowship
- Lee Kuan Yew Post-Doctoral Fellowship
- Singapore A*STAR
- NIH/NHGRI ENCODE Grant [HG004456]
Long-range chromatin contacts between specific DNA regulatory elements play a pivotal role in gene expression regulation, and a global characterization of these interactions in the 3-dimensional (3D) chromatin structure is imperative in understanding signaling networks and cell states. Chromatin Interaction Analysis using Paired-End Tag sequencing (ChIA-PET) is a method which converts functional chromatin structure into millions of short tag sequences. Combining Chromatin Immunoprecipitation (ChIP), proximity ligation and high-throughput sequencing, ChIA-PET provides a global and unbiased interrogation of higher-order chromatin structures associated with specific protein factors. Here, we describe the detailed procedures of the ChIA-PET methodology, unraveling transcription-associated chromatin contacts in a model human cell line. (C) 2012 Elsevier Inc. All rights reserved.
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