期刊
METHODS
卷 53, 期 2, 页码 175-184出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2010.07.006
关键词
DNA-methylation; Methylated DNA immunoprecipitation; (MeDIP); Mircoarrays; Quantitative real time PCR (qPCR); MeDIP-chip quality control; Enrichment of methylated regions; High-density tiling arrays
资金
- BMBF [01GS08112, 0315417D]
Genome-wide mapping of epigenetic changes is essential for understanding the mechanisms involved in gene regulation during cell differentiation and embryonic development. DNA-methylation is one of these key epigenetic marks that is directly linked to gene expression is. Methylated DNA immunoprecipitation (MeDIP) is a recently devised method used to determine the distribution of DNA-methylation within functional regions (e.g., promoters) or in the entire genome robustly and cost-efficiently. This approach is based on the enrichment of methylated DNA with an antibody that specifically binds to 5-methyl-cytosine and can be combined with PCR, microarrays or high-throughput sequencing. This article outlines the experimental procedure of MeDIP-chip and provides a comprehensive summary of quality control strategies and primary data analysis. (C) 2010 Elsevier Inc. All rights reserved.
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