期刊
METHODS
卷 52, 期 2, 页码 173-179出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2010.08.008
关键词
Tb3+; Luminescence; FRET; RNA
资金
- NSF [0316494, 0929394]
- National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH) [RR003037]
- Direct For Biological Sciences [0929394] Funding Source: National Science Foundation
Lanthanide ions such as Tb3+ and Eu3+ have long been used to probe RNA and protein structures due to their luminescence properties and their steric and chemical similarities to biological metal ions such as Mg2+ and Ca2+. In this article, we introduce a method that utilizes the enhanced Tb3+ luminescence upon site-binding to RNA molecules as a FRET donor. Using this method, it is possible to identify specific metal ion-binding locations within a folded RNA molecule. Applications of this method include introducing FRET donors into molecules of interest by engineering RNA loops or bulges as Tb3+-binding pockets. (C) 2010 Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据