期刊
METHODS
卷 46, 期 2, 页码 97-103出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2008.07.002
关键词
Solid supported membrane; Lipid membrane; Vesicle; Proteoliposome; Assay technique; Transport protein; Solution exchange; Capacitive coupling; Drug discovery
An assay technique for the electrical characterization of electrogenic transport proteins on solid supported membranes is presented. Membrane vesicles, proteoliposomes or membrane fragments containing the transporter are adsorbed to the solid supported membrane and are activated by providing a substrate or a ligand via a rapid solution exchange. This technique opens up new possibilities where conventional electrophysiology fails like transporters or ion channels from bacteria and from intracellular compartments. Its rugged design and potential for automation make it suitable for drug screening. (C) 2008 Elsevier Inc. All rights reserved.
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