期刊
METHODS
卷 46, 期 4, 页码 295-303出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2008.10.003
关键词
Mitochondrial fission; Mitochondrial fusion; Time-lapse imaging; Fluorescence wide-field microscopy; Motor neurons; Astrocytes; Mitochondrial length; Mitochondrial number
资金
- NIH [R01 EY016164 (EBW), R01 NS047456 (EBW), R01 NS055193 (EBW)]
Mitochondrial morphology and length change during fission and fusion and mitochondrial movement varies dependent upon the cell type and the physiological conditions. Here, we describe fundamental wide-field fluorescence microcopy and 3D imaging techniques to assess mitochondrial shape, number and length in various cell types including cancer cell lines, motor neurons and astrocytes. Furthermore, we illustrate how to assess mitochondrial fission and fusion events by 3D time-lapse imaging and to calculate mitochondrial length and numbers as a function of time. These imaging methods provide useful tools to investigate mitochondrial dynamics in health, aging and disease. (C) 2008 Elsevier Inc. All rights reserved.
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