期刊
METALLOMICS
卷 4, 期 3, 页码 253-259出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2mt00175f
关键词
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资金
- Swiss National Science Foundation [PZ00P2_121989]
- Forschungskredit of the University of Zurich [57010204]
The HEW lysozyme (Lys) and the fac-[Re(CO)(3)(H2O)(3)](+) complex (1) are used as a simple model system for the description of a new approach to the labelling polypeptides with fluorescent tags. The strategy takes advantage of the reaction of an acridine orange-based fluorophore (AO) with the non-native metal fragment 1 hybridized on the enzyme. A synthetic methodology for the quantitative metallation of the protein is first described and it is then shown that the exogenous metal complex can be exploited for the coupling of the fluorescent probe. All Lys-derived species were characterized by various spectroscopic techniques. It is shown that the approach does not significantly alter the activity of the final fluorescent metallo-protein conjugate (Lys2). The accumulation of Lys2 on Micrococcus lysodeikticus bacteria was observed via confocal laser scanning microscopy.
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