期刊
METABOLOMICS
卷 10, 期 4, 页码 543-555出版社
SPRINGER
DOI: 10.1007/s11306-013-0619-5
关键词
Oryza sativa; Rice; Tandem mass spectrometry (MS/MS); Nuclear magnetic resonance (NMR); Specialized metabolites; Flavonoid
资金
- Japan Science and Technology Agency (JST), Strategic International Collaborative Research Program (SICORP)
- Japan Advanced Plant Science Research Network
- Grants-in-Aid for Scientific Research [24590028, 25513012] Funding Source: KAKEN
Metabolomics plays an important role in phytochemical genomics and crop breeding; however, metabolite annotation is a significant bottleneck in metabolomic studies. In particular, in liquid chromatography-mass spectrometry (MS)-based metabolomics, which has become a routine technology for the profiling of plant-specialized metabolites, a substantial number of metabolites detected as MS peaks are still not assigned properly to a single metabolite. Oryza sativa (rice) is one of the most important staple crops in the world. In the present study, we isolated and elucidated the structures of specialized metabolites from rice by using MS/MS and NMR. Thirty-six compounds, including five new flavonoids and eight rare flavonolignan isomers, were isolated from the rice leaves. The MS/MS spectral data of the isolated compounds, with a detailed interpretation of MS fragmentation data, will facilitate metabolite annotation of the related phytochemicals by enriching the public mass spectral data depositories, including the plant-specific MS/MS-based database, ReSpect.
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