期刊
METABOLOMICS
卷 6, 期 3, 页码 427-438出版社
SPRINGER
DOI: 10.1007/s11306-010-0216-9
关键词
Metabolomics; Antibody; Glutamine synthetase; CHO; Bioprocessing; GC-MS
资金
- UK BBSRC
- EPSRC
- Bioprocessing Research Industry Club (BRIC)
- Biotechnology and Biological Sciences Research Council [BB/E005985/1, BB/G023581/2, BB/G023581/1] Funding Source: researchfish
- BBSRC [BB/E005985/1, BB/G023581/2, BB/G023581/1] Funding Source: UKRI
In this study we report on the optimisation of the technologies for generation of a global metabolomics profile for intracellular metabolites in Chinese hamster ovary (CHO) cells. We evaluated the effectiveness of a range of different extraction methods applied to CHO cells which had been quenched using a previously optimised approach. The extraction methods tested included cold methanol, hot ethanol, acid, alkali and methanol/chloroform plus combinations of these. The extraction of metabolites using two 100% methanol extractions followed by a final water extraction recovered the largest range of metabolites. For the majority of metabolites, extracts generated in this manner exhibited the greatest recovery with high reproducibility. Therefore, this was the best extraction method for attaining a global metabolic profile from a single sample. However, another parallel extraction method (e.g. alkali) may also be required to maximise the range of metabolites recovered (e.g. non-polar metabolites).
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