期刊
METABOLOMICS
卷 7, 期 2, 页码 257-269出版社
SPRINGER
DOI: 10.1007/s11306-010-0249-0
关键词
Stable isotope tracers; SIRM; SCID mouse; Metabolomics; Non-small cell lung cancer xenograft
资金
- National Science Foundation [EPS-0447479]
- NIH from the National Center for Research Resources [P20RR018733]
- National Cancer Institute [1R01CA118434-01A2, R01CA-086412, RO1 CA150947]
- Kentucky Challenge for Excellence
- Susan G. Komen Foundation [BCTR0503648]
- NCI [R21CA133688]
- Brown Foundation
We have determined the time course of [U-(13)C]-glucose utilization and transformations in SCID mice via bolus injection of the tracer in the tail vein. Incorporation of (13)C into metabolites extracted from mouse blood plasma and several tissues (lung, heart, brain, liver, kidney, and skeletal muscle) were profiled by NMR and GC-MS, which helped ascertain optimal sampling times for different target tissues. We found that the time for overall optimal (13)C incorporation into tissue was 15-20 min but with substantial differences in (13)C labeling patterns of various organs that reflected their specific metabolism. Using this stable isotope resolved metabolomics (SIRM) approach, we have compared the (13)C metabolite profile of the lungs in the same mouse with or without an orthotopic lung tumor xenograft established from human PC14PE6 lung adenocarcinoma cells. The (13)C metabolite profile shows considerable differences in [U-(13)C]-glucose transformations between the two lung tissues, demonstrating the feasibility of applying SIRM to investigate metabolic networks of human cancer xenograft in the mouse model.
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