期刊
METABOLIC ENGINEERING
卷 25, 期 -, 页码 1-7出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2014.06.003
关键词
Biofuel; Metabolic engineering; Fatty acid metabolism; Membrane composition
资金
- DOE grant [DE-FC02-02ER63421]
- U.S. Department of Energy (DOE) [DE-FC02-02ER63421] Funding Source: U.S. Department of Energy (DOE)
Microbial fatty acids are an attractive source of precursors for a variety of renewable commodity chemicals such as alkanes, alcohols, and bioluels. Rerouting lipid biosynthesis into free Fatty acid production can be toxic, however, due to alterations of membrane lipid composition. Here we find that membrane lipid composition can be altered by the direct incorporation of medium-chain Fatty acids into lipids via the Aas pathway in cells expressing the medium-chain thioesterase from Umbellularia californica (BTE). We find that deletion of the aas gene and sequestering exported fatty acids reduces medium-chain Fatty acid toxicity, partially restores normal lipid composition, and improves medium-chain Fatty acid yields. (C) 2014 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
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