期刊
METABOLIC ENGINEERING
卷 14, 期 5, 页码 496-503出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2012.07.003
关键词
PHB; Hydrogen production; Hydrogenase 3; Synthetic biology; Metabolic engineering; Escherichia coli
资金
- State Basic Science Foundation 973 [2012CB725200, 2012CB725201, 2011CBA00807]
- National Natural Science Foundation of China [31170099, 31070095]
Recombinant Escherichia coli was constructed for co-production of hydrogen and polyhydroxybutyrate (PHB) due to its rapid growth and convenience of genetic manipulation. In particular, anaerobic metabolic pathways dedicated to co-production of hydrogen and PHB were established due to the advantages of directing fluxes away from toxic compounds such as formate and acetate to useful products. Here, recombinant E. coli expressing hydrogenase 3 and/or acetyl-CoA synthetase showed improved PHB and hydrogen production when grown with or without acetate as a carbon source. When hydrogenase 3 was over-expressed, hydrogen yield was increased from 14 to 153 mmol H-2/mol glucose in a mineral salt (MS) medium with glucose as carbon source, accompanied by an increased PHB yield from 0.55 to 5.34 mg PHB/g glucose in MS medium with glucose and acetate as carbon source. (C) 2012 Elsevier Inc. All rights reserved.
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