期刊
METABOLIC ENGINEERING
卷 13, 期 5, 页码 492-498出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2011.05.003
关键词
5-aminolevulinic acid; Escherichia coli; Metabolic engineering; C5 pathway
资金
- National Natural Science Foundation of China [31070092]
- National Basic Research Program of China [2011CB707405]
- Graduate Independent Innovation Foundation of Shandong University (GIIFSDU) [yzc09048]
5-Aminolevulinic acid (ALA) recently received much attention due to its potential applications in many fields. In this study, we developed a metabolic strategy to produce ALA directly from glucose in recombinant Escherichia coli via the C5 pathway. The expression of a mutated hemA gene, encoding a glutamyl-tRNA reductase from Salmonella arizona, significantly improved ALA production from 31.1 to 176 mg/L. Glutamate-1-semialdehyde aminotransferase from E. coli was found to have a synergistic effect with HemA(M) from S. arizona on ALA production (2052 mg/L). In addition, we identified a threonine/homoserine exporter in E. coli, encoded by rhtA gene, which exported ALA due to its broad substrate specificity. The constructed E. coli DALA produced 4.13 g/L ALA in modified minimal medium from glucose without adding any other co-substrate or inhibitor. This strategy offered an attractive potential to metabolic production of ALA in E. coli. (C) 2011 Elsevier Inc. All rights reserved.
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