期刊
METABOLIC ENGINEERING
卷 11, 期 6, 页码 355-366出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2009.07.004
关键词
Flavonoids; Isoflavonoids; Stilbenoids; Metabolic engineering; Metabolic reconstitution; Plant biosynthesis; Genistein; Kaempferol; Naringenin; Quercetin; Resveratrol
资金
- General Secretariat Research & Technology (GSRT) of Greece [03ED 776]
- E. U.-European Social Fund
- Greek Ministry of Development-GSRT
- EPEAEK
- University Student Entrepreneurship
Chemical or biological synthesis of plant secondary metabolites has attracted increasing interest due to their proven or assumed beneficial properties and health promoting effects. Resveratrol, a stilbenoid, naringenin, a flavanone, genistein, an isoflavone, and the flavonols kaempferol and quercetin have been shown to possess high nutritional and agricultural value. Four metabolically engineered yeast strains harboring plasmids with heterologous genes for enzymes involved in the biosynthesis of these compounds from phenylalanine have been constructed. Time course analyses of precursor utilization and end-product accumulation were carried out establishing the production of 0.29-0.31 mg/L of trans-resveratrol, 8.9-15.6 mg/L of naringenin, 0.1-7.7 mg/L of genistein, 0.9-4.6 mg/L of kaempferol and 0.26-0.38 mg/L of quercetin in defined media under optimal growth conditions. The recombinant yeast strains can be used further for the construction of improved flavonoid- and stilbenoid-overproducers. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据