期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 106, 期 7, 页码 837-843出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762011000700009
关键词
Schistosoma mansoni; diagnosis; antigen candidate; bioinformatics analysis
资金
- RIPAG/FIOCRUZ-MG
- PAPES V/FIOCRUZ
- CPqRR/FIOCRUZ-MS
- FAPEMIG
- PV/CNPq
- PQ/CNPq
The development of a more sensitive diagnostic test for schistosomiasis is needed to overcome the limitations of the use of stool examination in low endemic areas. Using parasite antigens in enzyme linked immunosorbent assay is a promising strategy, however a more rational selection of parasite antigens is necessary. In this study we performed in silico analysis of the Schistosoma mansoni genome, using SchistoDB database and bioinformatic tools for screening immunogenic antigens. Based on evidence of expression in all parasite life stage within the definitive host, extracellular or plasmatic membrane localization, low similarity to human and other helminthic proteins and presence of predicted B cell epitopes, six candidates were selected: a glycosylphosphatidylinositol-anchored 200 kDa protein, two putative cytochrome oxidase subunits, two expressed proteins and one hypothetical protein. The recognition in unidimensional and bidimensional Western blot of protein with similar molecular weight and isoelectric point to the selected antigens by sera from S. mansoni infected mice indicate a good correlation between these two approaches in selecting immunogenic proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据