期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 103, 期 2, 页码 172-179出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762008000200008
关键词
Rhodnius prolixus; Trypanosoma rangeli KP1(+); Trypanosoma rangeli KP1(-); trypanolytic factor; vectorial ability; insect immunity
Rhodnius prolixus is the main Trypanosoma rangeli vector in several Latin-American countries and is susceptible to infection with KP1(+) strains; however, it presents an invasion-resistant response to KP1(-) strains. The present work has identified a trypanolytic protein against T. rangeli KP1(-) in the R. prolixus hemolymph which was fractioned with ammonium sulfate (following dialysis). The results revealed a protein component which did not depend on divalent cations for its biological function whilst keeping its trypanolytic activity at temperatures ranging from -20 degrees C to 37 degrees C, at 7.0 to 10.5 pH. The protein was partially purified by gel filtration chromatography and ionic exchange chromatography. The major component presented a molecular weight of around 79 kDa and an isoelectric point between 4.9 and 6.3 and may be directly related to hemolymph trypanolytic activity against T. rangeli KP1(-) populations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据