期刊
MEDICAL SCIENCE MONITOR
卷 18, 期 11, 页码 BR450-BR460出版社
INT SCIENTIFIC LITERATURE, INC
DOI: 10.12659/MSM.883538
关键词
meningioma; human cerebrospinal fluid; proteome; biomarker; brain tumor
资金
- Brain Research Center, The 21st Century Frontier Research Program of the Ministry of Education, Science and Technology, Republic of Korea [2009K001266]
- National Research Foundation of Korea
- Korean Government (MEST) (University-Institute cooperation program)
- Korea Basic Science Institute project Operation of the Advanced Multi-purpose Mass Spectrometers [G30124]
- Korean Government (MEST) (University-Institute cooperation program)
Background: To identify meningioma-specific proteins, cerebrospinal fluid (CSF) from 4 patients with a meningioma and 4 patients with a non-brain tumorous lesion were analyzed. Material/Methods: Two-dimensional electrophoresis and electrospray quadrupole time-of-flight tandem mass spectrometry analyses revealed 10 unique spots, containing 11 independent proteins (spot #2 and #4 each contained 2 proteins and spot #3 was not identified) were evident in CSF associated with human meningioma: serum albumin precursor (3 different isoforms), Apolipoprotein E (Apo E), Apolipoprotein J precursor (Apo J), Transthyretin precursor (TTR), Prostaglandin D2 synthase 21kDa (PTGDS), proapolipoprotein, Chain D hemoglobin Ypsilanti, alpha-1-antitrypsin (AAT), and beta-2-microglobulin precursor (beta 2M). Results: The contents of Apo E, Apo J and AAT were increased, while PTGDS, TTR and beta 2M were decreased. Conclusions: The results observed by 2-dimensional electrophoresis were verified by Western blot analysis. The unique proteins may represent possible candidate biomarkers of meningioma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据