期刊
RNA
卷 21, 期 8, 页码 1393-1395出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.051797.115
关键词
MS2-MCP system; mRNA decay; mRNA localization
资金
- National Institutes of Health [R01GM045443, F32 GM108075-01A1]
- Howard Hughes Medical Institute
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [F32GM108075, R01GM045443] Funding Source: NIH RePORTER
Arrays of MS2 binding sites are placed into mRNAs and are commonly used to visualize the localization of mRNAs in vivo by the expression of an MS2-GFP fusion protein. In Saccharomyces cerevisiae, we observed that arrays of MS2 binding sites inhibit 5' to 3' degradation of the mRNA in yeast cells and lead to the accumulation of a 3' mRNA fragment containing the MS2 binding sites. This accumulation can be dependent on the binding of the MS2 stem loops (MS2-SL) by MS2 coat proteins (MCPs). Since such decay fragments can still bind MCP-GFP, the localization of such mRNA fragments can complicate the interpretation of the localization of full-length mRNA in vivo.
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