期刊
RNA BIOLOGY
卷 13, 期 1, 页码 68-82出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2015.1119363
关键词
nocturnin; Arabidopsis; Allosteric; circadian; RNA stability; deadenylation
资金
- Department of Biochemistry and Biotechnology [3439, 3817]
We report the identification and characterization of a novel gene, AtHesperin (AtHESP) that codes for a deadenylase in Arabidopsis thaliana. The gene is under circadian clock-gene regulation and has similarity to the mammalian Nocturnin. AtHESP can efficiently degrade poly(A) substrates exhibiting allosteric kinetics. Size exclusion chromatography and native electrophoresis coupled with kinetic analysis support that the native enzyme is oligomeric with at least 3 binding sites. Knockdown and overexpression of AtHESP in plant lines affects the expression and rhythmicity of the clock core oscillator genes TOC1 and CCA1. This study demonstrates an evolutionary conserved poly(A)-degrading activity in plants and suggests deadenylation as a mechanism involved in the regulation of the circadian clock. A role of AtHESP in stress response in plants is also depicted.
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