期刊
MEDICAL ONCOLOGY
卷 31, 期 1, 页码 -出版社
HUMANA PRESS INC
DOI: 10.1007/s12032-013-0785-3
关键词
Laryngeal squamous cell carcinoma; MiR-205; Bcl-2; Proliferation; Apoptosis
类别
资金
- Heilongjiang Postdoctoral Fund [LBH-Z12157]
- foundation of Heilongjiang Educational Committee [12531343]
- foundation of Heilongjiang Health Bureau [2012-624]
- Youth Foundation of the Second Affiliated Hospital of Harbin Medical University [QN2011-01]
- National science Foundation of china [81241085, 81372902, 81272965]
- key project of Natural Science Foundation of Heilongjiang Province of China [ZD201215/H1302]
- Research Fund for the Doctoral Program of Higher Education of China [20102307110007]
- science and technology innovation talent research funds of Harbin [2012RFXXS072]
MicroRNAs were reported to be involved in the modulation of tumor development. The aim of our study was to investigate the effect of miR-205 on proliferation and apoptosis of laryngeal squamous cell carcinoma (LSCC) and seek associations between miR-205 and Bcl-2 using in vitro and in vivo methods. Real-time qPCR was used to analyze the expression of miR-205 in LSCC samples and Hep-2 cell line. Apoptosis, cell cycle, and proliferation (MTT) assays were performed to test the apoptosis and proliferation of LSCC cells after miR-205 transfection. Bcl-2 expression in cells was assessed with Western blotting. The tumorigenicity of LSCC cells was evaluated in nude mice model. MiR-205 was significantly down-regulated in LSCC tissues compared to adjacent normal tissues. Lower expression of miR-205 was indicated to be statistically related with advanced clinical stage and T3-4 grades. We found that restoration of miR-205 down-regulated the proliferative markers of dihydrofolate reductase and proliferating cell nuclear antigen and apoptotic regulator of Bcl-2. The findings in vitro and in vivo showed miR-205 could suppress cell proliferation and induce cell apoptosis. In addition, Bcl-2 was identified as one of the direct targets of miR-205 in LSCC cells. These results suggest that miR-205 may play as a tumor suppressor in LSCC, probably by targeting Bcl-2 and serve as a potential target for therapeutic intervention.
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