期刊
MEDICAL MYCOLOGY
卷 49, 期 1, 页码 73-79出版社
OXFORD UNIV PRESS
DOI: 10.3109/13693786.2010.507604
关键词
Aspergillus; NASBA; FRET
资金
- Ministry for Health, Welfare & Family Affairs, Republic of Korea [A080696]
We attempted to apply fluorescence resonance energy transfer technology to nucleic acid sequence-based amplification (FRET-NASBA) on the platform of the LightCycler system to detect Aspergillus species. Primers and probes for the Aspergillus 18S rRNA were newly designed to avoid overlapping with homologous sequences of human 18s rRNA. NASBA using molecular beacon (MB) showed non-specific results which have been frequently observed from controls, although it showed higher sensitivity (10
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