期刊
MEDICAL MYCOLOGY
卷 49, 期 5, 页码 458-466出版社
OXFORD UNIV PRESS
DOI: 10.3109/13693786.2010.535031
关键词
IPT1 gene; Sphingolipid; Candida; epithelial cells; oral mucosa; TLRs; defensins
资金
- BRS-ACURE [Q0600136]
- NIH/NIAID [R21-AI74077-01]
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- [NIH/R01-DE017486-01A1]
We demonstrated the effect of a Candida albicans sphingolipid biosynthetic gene, IPT1, on the interaction between gingival epithelial and Candida cells using monolayer cultures and engineered human oral mucosa tissue (EHOM). Disrupting the IPT1 gene greatly reduced Candida adhesion to gingival epithelial cells, compared to the wild-type and revertant strains. The yeasts adhesion to epithelial cells may activate toll-like receptors (TLRs). Cell response against Candida infection was thus investigated by evaluating TLR expression and antimicrobial peptide (AMP) production. The wild-type and revertant strains both activated TLR2, TLR4, TLR6, and TLR9 gene expression in the epithelial cells, whereas the delta Delta ipt1 mutant Candida strain had no effect on this expression. This finding was supported by an increased AMP expression (human beta beta-defensin HBD-2 and HBD-3) in the EHOM tissue infected with the wild-type and revertant Candida strains, and a decreased expression in the delta Delta ipt1 mutant-infected model. HBD protein secretion confirmed the absence of any effect by the delta Delta ipt1 on epithelial cell innate defense. This is the first study to demonstrate that a disruption of the IPT1 gene affects Candida-host interaction, thus preventing TLR activation and beta beta-defensin expression.
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