期刊
MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
卷 48, 期 10, 页码 1055-1063出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11517-010-0668-0
关键词
Malaria; Plasmodium falciparum; FLIM; FRET; Optical tweezers; Optical stretcher; Haemoglobin concentration; X-ray microanalysis; Micropositioning; EDS; EPXMA; Red cell model; AFM
类别
资金
- EPSRC [EP/E059384]
- BBSRC [BB/E008542/1]
- Isaac Newton Trust
- Korea Foundation for International Cooperation of Science & Technology (KICOS) through Korean Ministry of Education Science & Technology (MEST) [2009-00591]
- Engineering and Physical Sciences Research Council UK [EP/F044011/1]
- BBSRC [BB/E008542/1] Funding Source: UKRI
- EPSRC [EP/F044011/1, EP/F044011/2] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E008542/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/F044011/1, EP/F044011/2] Funding Source: researchfish
Investigation of the homeostasis of red blood cells upon infection by Plasmodium falciparum poses complex experimental challenges. Changes in red cell shape, volume, protein, and ion balance are difficult to quantify. In this article, we review a wide range of optical techniques for quantitative measurements of critical homeostatic parameters in malaria-infected red blood cells. Fluorescence lifetime imaging and tomographic phase microscopy, quantitative deconvolution microscopy, and X-ray microanalysis, are used to measure haemoglobin concentration, cell volume, and ion contents. Atomic force microscopy is briefly reviewed in the context of these optical methodologies. We also describe how optical tweezers and optical stretchers can be usefully applied to empower basic malaria research to yield diagnostic information on cell compliance changes upon malaria infection. The combined application of these techniques sheds new light on the detailed mechanisms of malaria infection providing potential for new diagnostic or therapeutic approaches.
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