期刊
MEDIATORS OF INFLAMMATION
卷 2009, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2009/193970
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资金
- Canadian Institutes of Health Research (CIHR) [MOP-89534]
- Fonds de la Recherche en Sante du Quebec (FRSQ)
Interleukin (IL)-4 is a cytokine known mainly for its anti-inflammatory activity. Using the in vivo murine air pouch model, we found that IL-4 significantly increased the number of leukocytes after 9 hours of treatment, consisting mainly of neutrophil (60%) and monocytic (40%) cell populations. Using an antibody array, we found that the expression of several analytes (predominantly CCL2) was increased by IL-4 before the arrival of leukocytes. The IL-4-induced expression of CCL-2 was confirmed by ELISA. Air pouch resident lining cells were harvested and were found to express IL-4R alpha. CCL2 mRNA expression was monitored in lining cells, cells isolated from the air pouch skin, in RAW264.7 macrophage and in epithelial Mode-K cells and its expression was increased in response to IL- 4 in all conditions. We conclude that IL-4 can attract leukocytes in vivo by an indirect mechanism involving the production of several analytes by, at least, resident cells. Copyright (C) 2009 Claude Ratthe et al.
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