期刊
MECHANISMS OF DEVELOPMENT
卷 126, 期 5-6, 页码 324-336出版社
ELSEVIER
DOI: 10.1016/j.mod.2009.02.006
关键词
SRY; SOX9; FOXL2; Sex determination; Gonad differentiation
资金
- Network in Genes and Environment in Development (NGED)
- Australian Research Council (ARC)
- National Health and Medical Research Council of Australia
- National Institutes of Health (USA) [DW (HD049431), EE (GM20919, RRO1183)]
- Australian Cancer Research Foundation/institute for Molecular Bioscience Dynamic Imaging Facility for Cancer Biology
Ovotestis development in B6-XYPOS mice provides a rare opportunity to study the interaction of the testis- and ovary-determining pathways in the same tissue. We studied expression of several markers of mouse fetal testis (SRY, SOX9) or ovary (FOXL2, Rspo1) development in B6-XYPOS ovotestes by immunofluorescence, using normal testes and ovaries as controls. In ovotestes, SOX9 was expressed only in the central region where SRY is expressed earliest, resulting in testis cord formation. Surprisingly, FOXL2-expressing cells also were found in this region, but individual cells expressed either FOXL2 or SOX9, not both. At the poles, even though SOX9 was not up-regulated, SRY expression was down-regulated normally as in XY testes, and FOXL2 was expressed from an early stage, demonstrating ovarian differentiation in these areas. Our data (1) show that SRY must act within a specific developmental window to activate Sox9; (2) challenge the established view that SOX9 is responsible for down-regulating Sry expression; (3) disprove the concept that testicular and ovarian cells occupy discrete domains in ovotestes; and (4) suggest that FOXL2 is actively suppressed in Sertoli cell precursors by the action of SOX9. Together these findings provide important new insights into the molecular regulation of testis and ovary development. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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